![]() Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10250 kD). Under suggested conditions, 10-245kDa Prestained Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. The ladder is supplied in gel loading buffer and is ready to use.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Trisphosphate, pH 7.5 at 25☌), 2 % SDS, 3.6 M Urea,1 mM 2-Mercaptoethanol, and 15 % (v/v) Glycerol). The 10-245 kDa Prestained Protein Ladderis designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. An extra tidbit: After you have done your Western blot visualization, stain the blot with amido black: Stain: 0. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading. Use a pre-stained protein ladder to track transfer of proteins from a gel to a membrane. The Western Blot is probed with a secondary antibody combination of Eu-streptavidin and the Eu-labeled secondary antibody required by your primary antibody. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Protein ladders and standards for SDS-PAGE, western blots, and isoelectric focusing (IEF) Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. The ScanLater Western Blot Protein Ladder Reagent contains seven biotinylated proteins in the molecular weight range of 10 kD to 140 kD to be detected using Eu-labeled streptavidin. The10-245 kDa Prestained Protein Ladderis a three-color protein standard with 12 pre stained proteins covering a wide range molecular weights from 10 to 245 kDa. The WesternSure chemiluminescent protein ladder is the only pre-stained, multi-colored protein ladder for both film and digital chemiluminescence detection. Apply more for thicker (> 1.5 mm) or larger gel.1.5~2.5 μl per well for general Western transferring.3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10 well mini-gel respectively. Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting.Features. ![]()
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